Novel carbosilane dendrimers for biomedical applications - interactions with biomolecules and biomembranes

01.01.2015 - 31.12.2017
Grant agency: 
Grant type: 
Standardní projekt
Registration number: 
Internal investigator/coinvestigator: 
Čermák Jan
Internal investigator: 
is coinvestigator only
External investigator/coinvestigator: 
J. E. Purkinje University, Faculty of Science

This project aims to introduce novel types of glycodendrimers based on carbosilane dendrimer core decorated with (oligo)sacharides and to investigate their interaction with model lipid membranes and biomolecules (anti-sense oligonucleotides, amyloid and prion peptides) by complex biophysical methods and computer modeling. A library of the dendrimers will be synthesized varying in generation, type of sugar shell (maltose, maltotriose, maltohexaose) and charge (negative, positive, neutral). The interaction of the dendrimers with the red blood cells, model lipid bilayers as well as cell lines will be studied with the aim to investigate their toxicity and the molecular mechanisms underlying this effect. Further on, their interaction with amyloids, model prionic peptides and antisense DNA molecules will be matter of research in order to define their potential biomedical applications.